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NeuroNexus is experienced in providing solutions for custom neural interfaces to meet diverse experimental needs. This portfolio shows some of our work to date.


Featured Custom Design: Dr. Rune Berg, University of Copenhagen

RuneBerg
A neuron should always be studied in context of a neuronal network. NeuroNexus helps provide the tools. ~Dr. Rune Berg

 

Dr. Berg’s design is a modification of the Buzsaki probe with the electrode distance being larger to match the size and distance of neurons in the spinal cord as opposed to in the hippocampus. Having the contacts on the edges allows sampling from a larger volume of tissue, and sampling with multiple recording sites greatly improves spike sorting quality. Now, Dr. Berg and his lab can start unraveling the mystery of motor pattern generation in the spinal cord.

Berg64 probe:

Berg

 

recording from the Spinal Cord Using a Berg A8x8-5mm-200-160 probe:

Berg figure

 

Dr. Ingmar Schneider
Laurent Lab, MPI for Brain Research, Frankfurt, Germany

Schneider

Until I used the custom µECoG array, I never knew the precise extent of visually evoked activity, especially with this spatial and temporal resolution.

~Mr. Ingmar Schneider

The Laurent lab is interested in the behavior, dynamics and emergent properties of neural systems. Their efforts are focused on the cerebral cortex of turtles to facilitate the identification, mechanistic characterization and computational description of cortical functional principles. The aim of Ingmar’s work is to functionally characterize visually responsive areas in turtle dorsal cortex and to analyze their spatiotemporal dynamics in response to naturalistic visual stimulation. The size and flexible cabling of the µECoG array have been designed specifically to perform chronic recordings from the entire extent of dorsal cortex. Electrode density and package size have been carefully balanced to (i) achieve high electrode density (64 channels, pitch 500µm) and (ii) ensure connector compactness for chronic implantation, without restricting the animals’ natural behavior. These µECoG electrodes allow routine recording of spatiotemporal patterns of both stimulus-evoked and spontaneous oscillatory activity in chronically implanted turtles.

Results

Chronic experiments are currently underway.

Probe Status

This electrode design is available in the catalog as a special order. (Contact us for more detail.)

Schneider FeatureProbe

A computer Rendering of Mr. Schneider's Custom Probe, the E64-500-20-60.



 

Dr. Jonathan Whitlock
Kavli Institute for Systems Neuroscience
Trondheim, Norway

Whitlock Banner-01

It was a pleasure working with the knowledgeable staff at NeuroNexus during the probe design process. No detail was spared in getting me a precisely engineered double-probe drive targeting exacting anatomical coordinates.

~Dr. Jonathan Whitlock

Dr. Whitlock has several years of experience conducting chronic in vivo single-unit recordings from multiple sites in freely-behaving animals (Whitlock et al., Neuron, 2012; Derdikman et al. Nat. Neurosci. 2009). One of the major challenges which NeuroNexus probes have helped solve for me, particularly when recording from cortical regions just beneath the skull, is low cell yield. The 15 micron thick shanks with tetrode-style recordings sites at the tips has indeed led to more cells recorded per animal, and every aspect of the design was optimized for my application. The other major challenge is in packing 16 tetrodes into separate, but close sectors of cortex– an arrangement where traditional drives were prohibitively bulky. Again, the engineers at NeuroNexus tackled the problem with a beautiful and simple design: two independent drives were positioned orthogonally in the same housing, and fit on the skull as a single piece. We are now able to consistently target 16 tetrodes(across 2 drives) to the same cortical areas and get units from every animal.

Results

Experiments are currently underway.

Probe Status

This electrode design is available in the catalog as a special order. (Contact us for more detail.)

Whitlock DualDrive-01

Custom dual-dDrive

 

A8x1tet 2mm200 160-01

A computer Rendering of the A8x1-tet-2mm-200-160

Dr. Susumu Takahashi
Doshisha University, Japan

Takahashi

The outstanding engineers at NeuroNexus realized my ideal probe design. The silicon probes with high density contacts made my experiments much finer and convincing.

~Dr. Susumu Takahashi

For several years, Dr. Takahashi has focused on the detailed information on action potentials in extracellular recordings using custom-made microwire electrode (‘Dodecatrode,’ Takahashi & Sakurai, Neurosci. 2005; Eur. J. Neurosci. 2007; Front. Neural Circuits. 2009). The major limitation of the microwire is that the arrangement of contacts in the brain is largely unknown. To overcome this limitation, Dr. Takahashi worked with NeuroNexus to realize a custom probe that has exceptionally high density contacts that fully cover a pyramidal cell layer of the hippocampal CA1. This custom probe in conjunction with customized software will enable Dr. Takahashi to examine the details of extracellular activity originating from soma, dendrites, and axons in freely behaving animals.

Results

Experiments are currently underway.

Probe Status

This electrode design is available in the catalog as a special order. (Contact us for more detail.)

Takahashi FeatureProbe

A computer Rendering and close-up View of the A2x32-10mm-dia-200-100



 

Drs. Hong Lei and Cécile Faucher
Washington University, USA

HongLei Faucher
Having such a high number of recording sites in 2 locations will significantly increase the chance to detect synchronous activity in one region of the moth brain and observe correlated responses in the target region.~Drs. Hong Lei & Cécile Faucher

The University of Arizona group is interested in how olfactory information is processed in insect brains, more particularly how synchronous activity in the antennal lobes is detected by downstream protocerebral neurons. They therefore needed to record simultaneously from two different locations in the small moth brain. NeuroNexus was tasked to design a high density probe to allow access to such a small brain region without excessive damage. A 64-channel array was designed that was comprised of a 3D stack of three 16-channel A-Probes and a single 16-channel A-Probe with flexible cable attached. The two units were integrated to one single connector, allowing access to two brain regions while minimizing the bulk of the probe assembly.

ResultS

Antennal lobe output neurons converge onto lateral 
protocerebrum, resulting in less specific responses at this synaptic level. The correlation between such converging inputs and responsiveness of protocerebral neurons is currently being characterized.

Probe Status

3D arrays are customizable by choosing any set of 2D arrays to fit your experimental needs.

probe-display

Schematic Representation of a NeuroNexus 3D Probe in the Moth Brain

probe-detail

Simultaneous Recordings from the Antennal Lobes (upper 5 traces) and Protocerebrum (lower trace)

probe-graph

A session of recordings from the 3D Probe Assembly