Science Update

Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals Featured

Publication Details

Featured Authors

Antal Berényi

György Buzsaki

Journal Name
J Neurophysiology
Experiment Type
Chronic Microdrive
Experiment Type
Animal Model
Brain Region
Cortex Neo
Brain Region
Brain Signal
Single Unit
Brain Signal
Product Category
  • Penetrating Electrode
Probe Detail/Package
Monitoring representative fractions of neurons from multiple brain circuits in behaving animals is necessary for understanding neuronal computation. Here, we describe a system that allows high-channel-count recordings from a small volume of neuronal tissue using a lightweight signal multiplexing headstage that permits free behavior of small rodents. The system integrates multishank, high-density recording silicon probes, ultraflexible interconnects, and a miniaturized microdrive. These improvements allowed for simultaneous recordings of local field potentials and unit activity from hundreds of sites without confining free movements of the animal. The advantages of large-scale recordings are illustrated by determining the electroanatomic boundaries of layers and regions in the hippocampus and neocortex and constructing a circuit diagram of functional connections among neurons in real anatomic space. These methods will allow the investigation of circuit operations and behavior-dependent interregional interactions for testing hypotheses of neural networks and brain function.
Berenyi et. al. "Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals." J Neurophysiol. 2014 Mar; 111(5):1132-49. doi: 10.1152/jn.00785.2013
United States
New York University Neuroscience Institute